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A Comparison Between Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay Methods for Detecting Leptospira in Equine Recurrent Uveitis
Gholam Ali Kojouri, DVM, PhDa
, Camellia Taghadosi, DVMb, Hassan Momtaz, DVM, PhDc, Ehsan Taheri, DVMd
Abstract
Leptospirosis is a widespread zoonotic disease that affects humans and many species of animals. Leptospiral organisms have long been presumed to be associated with the presence of equine recurrent uveitis (ERU). The connection between ERU and leptospirosis can be established using various techniques. In the current study, we used a polymerase chain reaction (PCR) assay to help establish a diagnosis of ERU caused by Leptospira infection and compared the results with those of enzyme-linked immune assay (ELISA). A total of 31 and 30 serum samples were taken from horses with ERU (based on clinical diagnosis) and horses that were healthy, respectively, between June 2007 and December 2008. The results showed that from 31 affected horses, a total of seven and five samples were positive for leptospiral infection using PCR and ELISA, respectively, whereas there was no evidence of infection with Leptospira spp. in 30 serum samples of healthy horses. All of the positive samples in ELISA were also positive by PCR, whereas PCR detected two positive cases that were negative by ELISA. Although there was no significant difference between these two methods, it is apparent that PCR may be a more reliable tool for detecting the presence of leptospires in ERU.
Keywords: ELISA, Equine, Uveitis, Leptospirosis, PCR
a Department of Clinical Sciences, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
b Department of Clinical Science, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
c Department of Microbiology and Biotechnology, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
d Department of Clinical Science, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
Gholam Ali Kojouri, DVM, PhDa
, Camellia Taghadosi, DVMb, Hassan Momtaz, DVM, PhDc, Ehsan Taheri, DVMdAbstract
Leptospirosis is a widespread zoonotic disease that affects humans and many species of animals. Leptospiral organisms have long been presumed to be associated with the presence of equine recurrent uveitis (ERU). The connection between ERU and leptospirosis can be established using various techniques. In the current study, we used a polymerase chain reaction (PCR) assay to help establish a diagnosis of ERU caused by Leptospira infection and compared the results with those of enzyme-linked immune assay (ELISA). A total of 31 and 30 serum samples were taken from horses with ERU (based on clinical diagnosis) and horses that were healthy, respectively, between June 2007 and December 2008. The results showed that from 31 affected horses, a total of seven and five samples were positive for leptospiral infection using PCR and ELISA, respectively, whereas there was no evidence of infection with Leptospira spp. in 30 serum samples of healthy horses. All of the positive samples in ELISA were also positive by PCR, whereas PCR detected two positive cases that were negative by ELISA. Although there was no significant difference between these two methods, it is apparent that PCR may be a more reliable tool for detecting the presence of leptospires in ERU.
Keywords: ELISA, Equine, Uveitis, Leptospirosis, PCR
a Department of Clinical Sciences, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
b Department of Clinical Science, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
c Department of Microbiology and Biotechnology, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
d Department of Clinical Science, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
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